Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Cell viability assay of Gastrodia elata rhizome extract on intestinal cells (Caco-2). Data are expressed as mean ± SD (%) of 5 independent experiments, each performed in triplicate and normalised to the control (0%) line. p < 0.05 vs. control.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Viability Assay, Control

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Effects of individual extracts on cell viability in Caco-2 cells under dose–response conditions, and effects of their combination under time-dependent conditions (1–6 h). In ( A ), dose–response effects of Paeonia lactiflora root extract; in ( B ), dose–response effects of Morus alba leaf extract; and in ( C ), comparative effects of the combination ( Gastrodia elata + Morus alba + Paeonia lactiflora ) versus individual components. Gastrodia elata concentration (0.1 mg/mL) were based on published dose–response data as described in and reported in . Data are expressed as mean ± SD (%) from 5 independent experiments, each performed in triplicate and normalized to the control (0%). * p < 0.05 vs. control.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Concentration Assay, Control

Journal: Nutrients

Article Title: A Combination of Plant-Derived Extracts Modulates Nutrient-Responsive Metabolic Signalling in an In Vitro Gut–Liver–Adipose Model

doi: 10.3390/nu18091393

Figure Lengend Snippet: Evaluation of intestinal permeability in Caco-2 cells. ( A ) TEER values measured using EVOM3 voltohmmeter over time (1–6 h). ( B – D ) TJ protein levels (Claudin-1, Occludin, and ZO-1, respectively) were quantified by ELISA after 6 h of treatment. ( E ) Absorption rate over time (1–6 h), calculated according to the equation J = Jmax [C]/(Kt + [C]) using fluorescent probe. ( F ) GLP-1 secretion was measured after 6 h by ELISA kit. The botanical extracts used in this study were derived from Morus alba leaves, Paeonia lactiflora roots, and Gastrodia elata rhizomes. Data in panel ( A ) and ( F ) are presented as mean ± SD from five independent experiments each performed in triplicate. Data in panels ( B – E ) are expressed as mean ± SD from 5 independent experiments, each performed in triplicate and normalized to the control (0%).* p < 0.05 vs. control; α p < 0.05 vs. single components.

Article Snippet: Human intestinal epithelial Caco-2 cells and enteroendocrine NCI-H716 cells (American Type Culture Collection, ATCC ® , Manassas, VA, USA) were used to create an in vitro intestinal model that mimics the epithelial–enteroendocrine interface, allowing simultaneous evaluation of epithelial transport and GLP-1 secretion.

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Derivative Assay, Control